Metabolites Separation Services
There are uncountable products on the market with adverse side effects on health. Metabolites as natural products are considered an effective solution to reduce the use of unhealthy products[1]. Presently, metabolites have been utilized in various fields such as medicinal, colorant, aromatic and functional foods. In particular, metabolites play an important role in drug development and clinical diagnosis. However, metabolites are produced by the metabolic activity of people, plants, or microorganisms and are usually mixtures rather than pure substances. Metabolites separation is often the first step in the development and application of metabolites. The separation methods should be selected according to the physicochemical properties and biological stability of metabolites. A suitable separation method can reduce research costs and shorten the research period.
Alfa Chemistry has a team of highly skilled Ph.D. and M.S. biochemists with rich research and development experience as well as professional metabolites knowledge. At the same time, Alfa Chemistry have the world’s leading laboratories and advanced technical platforms for metabolites separation. We have the ability to provide customers with professional metabolites separation services.
Our Service Scope
Alfa Chemistry has extensive experience in the separation of various metabolites. Our metabolites separation services are as follows.
Alfa Chemistry offers human metabolites separation services, include human endogenous metabolites separation and human exogenous metabolites separation.
Alfa Chemistry offers plant metabolites separation services, include plant primary metabolites separation and plant secondary metabolites separation.
Alfa Chemistry offers microbial metabolites separation services, include microbial primary metabolites separation and microbial secondary metabolites separation.
Our Technical Platforms
Alfa Chemistry has established a number of technical platforms for metabolites separation, including but not limited to the following.
Partition chromatography is a technique based on the component partition of a mixture between stationary and mobile phases. It has various types such as paper chromatography, gas-liquid chromatography, liquid-liquid chromatography, etc. Partition chromatography has high separation efficiency and can separate metabolites in a short period. Many metabolites can be separated by partition chromatography such as alkaloids, vitamins, hormones, etc.
- Adsorption chromatography
Adsorption chromatography is a technique that involves the separation of a chemical mixture based on the interaction of the adsorbate with the adsorbent. The mixture of gas or liquid gets separated when it passes over an adsorbent bed that adsorbs different compounds at different rates. Adsorption chromatography includes thin layer chromatography, column chromatography, and gas-solid chromatography. Many typical primary metabolites can be separated by adsorption chromatography.
- Ion exchange chromatography
Ion exchange chromatography is a technique that separates ions and polar molecules based on their affinity to ion exchangers. This separation technique exploits the phenomenon of association between the ionizable molecules present in the sample and oppositely charged resins attached to the stationary phase. Ion exchange chromatography has the advantage of high resolution and heavy capacity. It is suitable for the separation of charged metabolites.
- Reverse phase chromatography
Reverse phase chromatography is a technique using alkyl chains covalently bonded to stationary phase particles in order to create a hydrophobic stationary phase. This stationary phase has a strong affinity for hydrophobic or less polar compounds. The use of a hydrophobic stationary phase is essentially the reverse of normal phase chromatography, since the polarity of the mobile and stationary phases has been inverted. The advantage of reverse phase chromatography is the ability to use the selectivity of pH to improve the separation.
Our Advantages
Alfa Chemistry has been deeply engaged in the field of metabolites separation for many years. Our advantages include, but are not limited to the following.
- Alfa Chemistry has competitive service prices to reduce your research costs.
- Alfa Chemistry has a professional technical team to provide high quality metabolites separation services.
- Alfa Chemistry has advanced high-resolution chromatogram platform that makes metabolites separation easy.
- Alfa Chemistry has reliable pre-sales and after-sales service that is responsible for the data to the end.
Please contact us if you have questions about metabolites separation. We will provide you with professional and high quality services.
Ordering Process
Reference
- Bajpai, V.K.; et al. Isolation and purification of plant secondary metabolites using column-chromatographic technique. Bangladesh Journal of Pharmacology. 2016, 11: 844-848.
Please kindly note that our products and services are for research use only.
