Metabolic Flux Analysis Services

Metabolic flux analysis (MFA), an important analytical technique, is used to examine the production and consumption rates of metabolites in a biological system. Over the past three decades, MFA has been widely used to quantify intracellular metabolic flux in both native (wild type) and engineered biological systems^[1]. With MFA, changes in metabolic flux caused by genetic and/or environmental interventions can be quantified. This information, in turn, provides insights into the regulation of metabolic pathways and may offers new targets for further microbial strains metabolic engineering.

Alfa Chemistry has a team of highly skilled Ph.D. and M.S. biochemists with rich research and development experience as well as professional metabolites knowledge. At the same time, Alfa Chemistry has the world's leading laboratories and advanced methods for MFA. We have ability to provide customers with MFA services of various biological systems.

Our Service Scope

Alfa Chemistry has rich MFA experience of biological systems. Our analysis scope includes, but are not limited to the following.

Our Analysis Methods

Alfa Chemistry has numerous methods for MFA, including stoichiometric MFA, ¹³C-MFA, isotopic non-stationary ¹³C MFA (¹³C-NMFA), dynamic MFA (DMFA), and ¹³C dynamic MFA (¹³C-DMFA). The main advantage of these methods is as follows.

• Stoichiometric MFA: The main advantage of stoichiometric MFA is its ease of application, which is accepted by many researchers. It requires only simple linear algebra and relies on relatively robust measurements of extracellular metabolites.
• ¹³C-MFA: The main advantage of ¹³C-MFA for quantifying flux is the fact that a large number of redundant measurements can be obtained for flux estimation. In addition, more complex metabolic network models can be investigated with ¹³C-MFA.
• ¹³C-NMFA: The main advantage of ¹³C-NMFA is that its metabolite pool sizes are fitted in ¹³C-NMFA to account for the observed labeling transients.
• DMFA: The main advantage of DMFA is that it does not depend on manually selecting time intervals to describe flux transients. Instead, rigorous statistical criteria is used to automatically detect the level of dynamic information present in the data.
• ¹³C-DMFA: The main advantage of ¹³C-DMFA is to incorporate isotope labeling measurements to allow for the estimation of cyclic pathways flux, parallel pathways flux, and reversible reactions flux.

Depending on your detailed requirements, Alfa Chemistry is able to choose the optimal MFA method for you.

Our Advantages

Alfa Chemistry has been deeply engaged in the field of MFA for many years. Our advantages include, but are not limited to the following.

• Alfa Chemistry has competitive service prices to reduce your research costs.
• Alfa Chemistry has a professional technical team to provide high quality MFA services.
• Alfa Chemistry has advanced MFA methods to make MFA data comprehensive and accurate.
• Alfa Chemistry has reliable pre-sales and after-sales service that is responsible for the data to the end.

Alfa Chemistry is proud to deliver customer-focused MFA services. Over the years, customers have turned to us not only for our expertise in innovation but for our reputation for agility and for delivering results on time and under budget. Please contact us if you have questions about MFA. We will provide you with professional and high quality services.

Ordering Process

Reference

1. Antoniewicz, M.R. Methods and advances in metabolic flux analysis: a mini-review. Journal of Industrial Microbiology & Biotechnology. 2015, 42: 317-325.

Please kindly note that our products and services are for research use only.